Effect of magnesium and osteoblast cell presence on hydroxyapatite formation on (Ti,Mg)N thin film coatings
Yükleniyor...
Dosyalar
Tarih
2016-07-19
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
TiN and (Ti,Mg)N thin film coatings were deposited on Ti substrates by an arc-physical vapor deposition technique. The effect of cell presence on hydroxyapatite (HA) formation was investigated using surfaces with four different Mg contents (0, 8.1, 11.31, and 28.49 at.%). Accelerated corrosion above 10 at.% Mg had a negative effect on the performance in terms of both cell proliferation and mineralization. In the absence of cells, Mg-free TiN coatings and low-Mg (8.1 at.%)-doped (Ti,Mg)N surfaces led to an early HA deposition (after 7 days and 14 days, respectively) in cell culture medium (DMEM), but the crystallinity was low. More crystalline HA structures were obtained in the presence of the cells. HA deposits with an ideal Ca/P ratio were obtained at least a week earlier, at day 14, in TiN and low-Mg (8.1 at.%)-doped (Ti,Mg)N compared with that of high-Mg-containing surfaces (>10 at.%). A thicker mineralized matrix was formed on low-Mg (8.1 at.%)-doped (Ti,Mg)N relative to that of the TiN sample. Low-Mg doping (<10 at.%) into TiN coatings resulted in better cell proliferation and thicker mineralized matrix formation, so it could be a promising alternative for hard tissue applications.
Açıklama
Anahtar Kelimeler
Cell culture, Cell proliferation, Cells, Coatings, Cytology, Deposition, Hard coatings, Hydroxyapatite, Physical vapor deposition, Semiconductor doping, Thin films, Tin deposits, Titanium compounds, Titanium nitride, Absence of cells, Accelerated corrosion, Arc physical vapor deposition, Cell culture mediums, Hydroxyapatite formations, Matrix formation, Osteoblast cells, Thin-film coatings, Vapor deposition, Pulsed-laser deposition, Calcium-phosphate, Substituted hydroxyapatite, In-vitro, Thermal-stability, Titanium surface, Dental implants, Bone, Adhesion, Behavior
Kaynak
JOM
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
69
Sayı
7
Künye
Önder, S., Çalıkoğlu Koyuncu, A. C., Torun Köse, G., Kazmanlı, K., Kök, F. N. & Ürgen, M. (2016). Effect of magnesium and osteoblast cell presence on hydroxyapatite formation on (ti,mg)N thin film coatings. JOM, 69(7), 1195-1205. doi:10.1007/s11837-016-2029-4
